1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

KM Value

KM Value on EC 1.1.1.1 - alcohol dehydrogenase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
(+)-2-methylcyclohexanone
-
-
-
9.1
(2E)-but-2-en-1-ol
pH 8.0, 55C
-
3.3
(2E)-but-2-enal
pH 6.5, 65C
2.4
(R)-1-indanol
65C, pH 8.0; pH 8.0, 65C
0.41 - 37.4
(R)-2-butanol
1.92
(R)-2-octanol
-
-
0.22 - 31.7
(R)-2-pentanol
0.02
(R)-2-phenylpropanol
-
-
1.28 - 1.3
(R)-alpha-tetralol
4.2
(S)-(+)-1-indanol
-
65C
4.2
(S)-(+)-alpha-tetraol
-
65C
18.1
(S)-(-)-1-phenylethanol
-
65C
4.2 - 6.4
(S)-1-indanol
18.1
(S)-1-phenylethanol
-
pH 10.5, 65C
0.012 - 120
(S)-2-butanol
0.18
(S)-2-octanol
-
-
0.07 - 8.8
(S)-2-pentanol
0.157
(S)-2-phenylpropanol
-
-
1.05 - 4.2
(S)-alpha-tetralol
8.47
(S)-N-benzyl-3-pyrrolidinol
-
pH 8.0
1.65 - 15.84
1,2-propanediol
26.7
1,3-Propanediol
-
pH 9.0, 60C, recombinant enzyme
0.08 - 230
1-butanol
0.000036 - 0.0038
1-formyl-6-methylpyrene
0.000035 - 0.00149
1-formyl-8-methylpyrene
0.0005 - 0.012
1-formylpyrene
0.038 - 0.67
1-Heptanol
0.035 - 0.88
1-Hexanol
0.00028 - 0.0064
1-hydroxymethyl-6-methylpyrene
0.00031 - 0.059
1-hydroxymethyl-8-methylpyrene
0.00059 - 0.076
1-hydroxymethylpyrene
5.1 - 8.7
1-Indanol
27.6
1-Indanone
-
65C; pH 10.5, 65C
0.025 - 3.88
1-Octanol
0.027 - 78
1-Pentanol
5.9
1-phenyl-1,2-propandione
-
pH 10.5, 65C
5 - 12.1
1-phenyl-1,2-propanedione
0.025 - 244
1-phenylethanol
0.24 - 6.5
1-propanol
0.2
11-cis-retinal
-
reduction with NADH
0.018 - 0.28
11-cis-retinol
0.013 - 2.3
12-hydroxydodecanoate
0.056
12-Hydroxydodecanoic acid
-
-
0.086 - 0.1
12-hydroxylauric acid methyl ester
-
0.066
12-oxolauric acid methyl ester
-
wild-type, pH 8.0, 60C
-
0.11 - 0.137
13-cis-retinal
0.257
13-cis-retinol
-
oxidation with NAD+
0.06
16-hydroxyhexadecanoate
-
-
11.9
2',3',4',5',6'-pentafluoroacetophenone
-
65C, pH 5.0
6.3 - 23.5
2,2,2-trifluoroacetophenone
0.11
2,2-dichloroacetophenone
-
65C, pH 5.0
61.3
2,3-Butanediol
pH 9.0, 70C
1.05 - 11.9
2-butanol
15
2-butanone
-
-
0.35 - 60
2-Buten-1-ol
0.147
2-decanone
-
pH 8.0, 60C
310
2-deoxy-D-ribose
-
-
0.25
2-ethoxyethanol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65C
0.0021 - 0.009
2-formylpyrene
1.16
2-Heptanone
-
pH 8.0, 60C
5.01
2-Hexanone
-
pH 8.0, 60C
0.00048 - 0.106
2-hydroxymethylpyrene
0.021
2-Methylcyclohexanol
-
-
2.7
2-methylpropan-1-ol
-
-
0.215
2-nonanone
-
pH 8.0, 60C
0.286
2-Octanone
-
pH 8.0, 60C
0.752
2-Pentanol
-
pH 8.0, 60C
5.15 - 14.2
2-Pentanone
0.864
2-phenylpropionaldehyde
-
-
0.6 - 560
2-propanol
0.025 - 0.026
3,4-dihydro-retinal
0.024 - 0.028
3,4-dihydro-retinol
0.14 - 0.9
3-bromobenzyl alcohol
0.14
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65C
4.4
3-Methoxybenzaldehyde
-
65C
1.2 - 3.3
3-Methoxybenzyl alcohol
1.2
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65C; in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65C
7.5
3-methylbutan-2-one
-
-
9.9
3-Methylcyclohexanol
65C, pH 8.0
66
3-methylcyclohexanone
65C, pH 5.5
0.031
3-oxo-5beta-androstan-17beta-ol
-
-
0.27 - 1.6
3-Pentanol
75
3-Pentanone
-
-
0.032
3-Phenyl-1-propanol
-
-
0.033
3-phenylpropanol
-
-
0.128 - 0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
0.149 - 0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
0.35
3beta,7alpha-12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 4
0.032 - 0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
0.12 - 0.4
4-bromobenzyl alcohol
0.12
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65C
0.28
4-carboxybenzaldehyde
0.14 - 1.11
4-chloroacetophenone
0.00048 - 0.0038
4-formylpyrene
0.004 - 0.015
4-hydroxy-retinol
0.00037 - 0.04
4-hydroxymethylpyrene
0.024 - 4.4
4-methoxybenzaldehyde
0.07 - 61
4-methoxybenzyl alcohol
1.3
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65C
0.131
4-methoxyphenylacetone
-
pH 8.0, 60C
6.7 - 53.8
4-Methyl-1-pentanol
0.81
4-methylpentan-1-ol
-
-
0.017 - 0.027
4-oxo-retinal
9.45
5-hydroxymethylfurfural
-
mutant S110P/Y295C, pH 6.7, 30C
0.0036 - 0.04
5alpha-androstan-17beta-ol-3-one
0.011 - 0.046
5beta-androstan-17beta-ol-3-one
0.123 - 0.164
5beta-androstan-3beta-ol-17 one
0.003 - 0.058
5beta-androstan-3beta-ol-17-one
0.008 - 0.25
5beta-cholanic acid-3-one
0.047
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
-
-
0.0036
5beta-pregnan-3,20-dione
-
pH 7.3, 37C, ADH1C*2 (gamma2gamma2)
0.027
5beta-Pregnan-3beta-ol-20-one
-
pH 7.3, 37C, ADH1C*2 (gamma2gamma2)
0.16 - 0.242
9-cis-retinal
0.011 - 0.3
9-cis-retinol
0.0086 - 121
acetaldehyde
6.5 - 30.2
acetoin
6.6 - 135
acetone
0.2 - 0.66
acetophenone
0.3 - 0.61
acycloNAD+
0.011 - 0.34
all-trans-retinal
0.009 - 0.059
all-trans-retinol
0.39
allyl alcohol
-
pH 7.3, 37C
5.3
alpha-tetralol
-
65C
5.8
alpha-tetralone
-
65C; pH 10.5, 65C
0.0025 - 0.09
Anisaldehyde
0.03 - 33
benzaldehyde
0.43
benzil
-
65C, pH 5.0
0.007 - 49
benzyl alcohol
0.0145 - 0.8
benzylalcohol
240 - 2290
beta-NAD+
0.055 - 5.65
butan-1-ol
86.8
Butan-2,3-diol
-
pH 8.8, 70C
0.35 - 93.3
butan-2-ol
14
butan-2-one
-
-
0.48 - 4.24
Butanal
0.012 - 53.9
butanol
0.06 - 0.27
Butyraldehyde
0.14 - 0.25
Capronaldehyde
0.03
cinnamaldehyde
-
pH 7.7, 60C
0.11 - 4.58
cinnamyl alcohol
1
cis-4-methylcyclohexanol
-
-
4.2
cis-decahydro-1-naphthol
65C, pH 8.0
0.71
crotyl alcohol
-
pH 7.3, 37C
5.1 - 9.3
cycloheptanol
0.008 - 1900
Cyclohexanol
1.39 - 14.1
cyclohexanone
1.3
Cyclopentanone
-
-
0.0172 - 5000
ethanol
16.6
ethyl 3-methyl-2-oxobutyrate
-
65C, pH 5.0
1 - 5.3
ethyl benzoylformate
1
ethyl oxo(phenyl)acetate
-
pH 10.5, 65C
-
5.3
ethyl pyruvate
-
pH 6.5, 50C, recombinant enzyme
50 - 290
ethylene glycol
4.46 - 50
formaldehyde
0.13 - 18.8
furfural
2.58 - 18.6
glycerol
0.012
hexaldehyde
-
-
0.0007 - 17
Hexanol
0.71
isatin
65C, pH 5.5; pH 5.5, 65C
2.4
iso-propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65C
0.81
isoborneol
-
65C, pH 10.5
0.26 - 0.76
Isobutyraldehyde
197
Isopentanol
at pH 7.0 and 25C
9 - 20.3
Isopropanol
1.4
m-nitrobenzaldehyde
-
isoenzyme ADH-1, pH 7.5
0.78 - 150
methanol
6.5
methyl benzoylformate
65C, pH 5.5
2.8
methyl o-chlorobenzoylformate
65C, pH 5.5; pH 5.5, 65C
2.7
methyl oxo(phenyl)acetate
-
pH 10.5, 65C
2.7
methylbenzoylformate
-
65C
35
methylglyoxal
-
-
0.13 - 0.58
N-benzyl-3-pyrrolidinone
0.03 - 35.1
n-butanol
0.02 - 0.26
n-Hexanol
10.3
n-Pentanal
-
33C, pH 8
0.19 - 7.49
n-Propanol
0.001 - 710
NAD+
0.0004 - 260
NADH
0.066 - 2.4
NADP+
0.6 - 0.84
NADPH
0.011
octan-1-ol
-
enzyme form ADH-3
0.043 - 9.6
octanal
0.0014 - 1.6
Octanol
2.9 - 5.9
p-Methoxybenzaldehyde
6.9 - 22
p-methoxybenzyl alcohol
0.2 - 4.1
pentan-1-ol
0.05
pentan-2-ol
-
-
0.225
pentan-3-ol
-
-
0.012 - 56.2
Pentanol
13.6
phenyl trifluoromethyl ketone
-
25C, pH 7.0
0.19 - 0.91
phenylacetaldehyde
24.45 - 37.55
phenylglyoxylic acid
0.019 - 1.9
propan-1-ol
0.5 - 193
propan-2-ol
0.59
propanal
-
33C, pH 7
0.91 - 43.9
Propanol
2.01
propanone
-
33C, pH 7
1.24
propionaldehyde
-
-
3.4
R-(+)-1-phenylethanol
-
-
1.2
R-(+)-cis-verbenol
-
-
1.5
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
-
0.011 - 0.012
retinol
3.9
S-(-)-1-phenylethanol
-
-
0.95
S-(-)-2-methylbutan-1-ol
-
-
3.8
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
-
0.85
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
0.694
tert-butyl acetoacetate
-
pH 8.0, 60C
8.5
tetralin-1-ol
-
65C, pH 10.5
1.1
trans-4-methylcyclohexanol
-
-
0.009 - 0.23
trans-cinnamaldehyde
0.2
tryptophol
-
-
0.17 - 2
Valeraldehyde
0.034 - 11
Vanillyl alcohol
additional information
additional information
-