1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

Cloned

Cloned on EC 1.1.1.1 - alcohol dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5-7 genes encoding ADH, DNA and amino acid sequence determination and analysis, polymorphism and allelic frequencies analysis, gene ADH2 possesses 2 allelic forms with Ile308 or Val308, expression of ADH2 alloenzymes in Escherichia coli
-
ADH1 is expressed in recombinant Escherichia coli; ADH3 is expressed in recombinant Escherichia coli
class III enzyme
-
class IV enzyme, expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme under the control of the strong constitutive Arxula adeninivorans-derived TEF1 promoter in auxotrophic Arxula adeninivorans strain G1214, Hansenula polymorpha strain RB11, and Saccharomyces cerevisiae strainSEY6210, using different expression modules for transformation, evaluation of effeciency, overview. Expression in Arxula adeninivorans is most effective
-
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli using a rhamnose-inducible system
-
DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged BmADH protein in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 (DE3) pLysS cells
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21DE3pLysS cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM109(DE3) cells
-
expressed in Escherichia coli RB791 cells
-
expressed in Escherichia coli strain RB791
-
expressed in Hep-G2 cells
-
expression in Caldicellulosiruptor bescii; expression in Caldicellulosiruptor bescii
expression in Clostridium thermocellum; expression in Clostridium thermocellum DSM 1313
expression in Escherichia coli
expression in Escherichia coli host strain PBL339
-
expression in Escherichia coli strain TG-1
-
expression in Escherichia coli. The correct folding of the AdhC enzyme from hyperthermophilic Pyrococcus furiosus in mesophilic recombinant Escherichia coli is greatly influenced by the cultivation temperature. When grown at temperatures above the optimal growth temperature, Escherichia coli produces heat shock proteins to prevent protein aggregation. Heat shock proteins are known for their chaperonin activity, i.e., they help the protein folding and are responsible for an efficient protein quality control. When heated at 45°C for 2.5 h prior to induction, an increase of the activity is monitored compared to the standard cultivation at 37°C. The fast increase to 42°C yields more active enzyme than the slow increase to 42°C. This suggests that heat shock proteins either assist in the correct folding of the AdhC, or maybe even allow for resolubilization of (partially) denatured molecules. The cultivation at 45°C is not successful
expression in Escherichia coli; expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
expression in Escherichia coli; overexpressed in Escherichia coli
expression in Pyrococcus furiosus from which the native aldehyde oxidoreductase (AOR) gene is deleted. A strain containing the Thermoanaerobacter ethanolicus AdhE in a synthetic operon with AdhA is constructed. The AdhA gene is amplified from Thermoanaerobacter sp. X514. The amino acid sequence of TxAdhA is identical to that of TeAdhA. Of the bacterial strains expressing the various heterologous AdhE genes, only those containing AdhE and AdhA from Thermoanaerobacter sp. produced ethanol above background. The Te-AdhEA strain containing both AdhE and AdhA produces the most ethanol (4.2 mM), followed by Te-AdhE (2.6 mM), AdhA (1.8 mM) and Tx-AdhE (1.5 mM). Ethanol and acetate are the only major carbon end-products from glucose under these conditions. For these four strains, the amount of ethanol produced per estimated glucose consumed is increased from the background level to 1.2, 1.0, 0.8 and 0.7 respectively
expression in yeast
-
expression of ADH1C*2 in Escherichia coli
-
expression of ADH4 in Escherichia coli
-
expression of human ADH1 in an in vitro transcription/translation system, N-terminally GST-tagged ADH1 in COS cells and in Escherichia coli
-
expression of isozymes in Escherichia coli strain BL21
expression of rat ADH5 in an in vitro transcription/translation system, GFP-tagged ADH5 in COS cells, but no soluble ADH5 protein from heterologously expression in Escherichia coli cells with expression systems successfully used for other mammalian ADHs, including fused to glutathione-S-transferase
-
expression of Tx-AdhE in Pyrococcus furiosus from with the native aldehyde oxidoreductase (AOR) gene is deleted. Ethanol and acetate are the only major carbon end-products from glucose under these conditions. The amount of ethanol produced per estimated glucose consumed is increased from the background level 0.7 respectively
gene adh3, sequence comparison, expression of N-terminally His-tagged enzyme in Escherichia coli strains NovaBlue Singles and M15[pREP4]
-
gene ADH3, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Echerichia coli strain BL21 (DE3), complementation of the HpADH3 mutant by an HpADH3 expression cassette fused to a strong constitutive promoter, the resulting strain produced a significantly increased amount of ethanol compared to the wild-type strain in a glucose medium, while in a xylose medium, the ethanol production is dramatically reduced in an HpADH3 overproduction strain compared to that in the wild-type strain, semi-quantitative RT-PCR analysis
gene chy1186, overexpression in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha
-
gene encoding for ADH of the haloalkaliphilic archaeon Natronomonas pharaonis, which has a 1,068-bp open reading frame that encodes a protein of 355 amino acids, is cloned into the pET28b vector and is expressed in Escherichia coli
-
gene GmAdh2, quantitative RT-PCR expression analysis, phylogenetic analysis
heterologously overexpressed in Escherichia coli
-
homologously expressed
mutant enzyme S109P/L116S/Y294
-
overexpressed in Escherichia coli
overexpression as GST-fusion protein in Escherichia coli
-
overexpression in Saccharomyces bayanus